| Abstract Detail
Recent Topics Posters Malhi, Mahadev [1]. Somatic Embryogenesis and Regeneration in Local Mungbean (Vigna Radiate L). In present study the callusing and regeneration response of four EMS and NaCl treated lines of two local mungbean cultivars NM-92and NM-98 were evaluated using shoot apical meristem, cotyledon, and leaf explants on MS medium supplemented with various concentrations of 2, 4-D and BAP and IBA. The highest callusing response was obtained in L2 when leaf and shoot epical meristem explants were used on MS medium supplemented with 1.809μM 2, 4-D and 3.555μM BAP while leaf explants of L1 and L2 lines produced maximum callus by fresh weight. The shoot apical meristem, cotyledon and leaf explants showed callusing response after seven days, ten days and one month respectively. The MS medium supplemented with 3.4μM IBA showed maximum regeneration in calli obtained from different lines.
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1 - Institute of Biotechnology and Genetic Engineering, University of Sind, Genetics, Institute of Biotechnology and Genetic Engineering, University of Sindh, Jamshoro, Pakistan, same, Jamshoro, Asia, +92, Pakistan
Keywords:
Embryo development
dev.
Presentation Type: Recent Topics Poster
Session: P
Location: Ball Room & Party Room/SUB
Date: Monday, July 28th, 2008
Time: 12:30 PM
Number: PRT016
Abstract ID:1144 |
