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Abstract Detail


Recent Topics Posters

Kuo, Li-Yaung [1], Li, Fay-Wei [2], Wang, Chun-Neng [2].

The first insight into fern matK phylogeny.

MatK gene, perhaps the most powerful chloroplast marker in angiosperms, has been massively exploited for various levels of phylogenetic studies, and even been proposed as the prime DNA barcode of plants. Such remarkable applicability results from its high substitution rate comparable to those of chloroplast non-coding regions, yet posing little ambiguous alignment problem. Nonetheless, matK among ferns have not been paid much attention and the utility remains vastly unknown.
Unlike angiosperms, the lack of conserve priming site in most ferns, the trnK exons, makes the amplification very challenging.
However, we have successfully sequenced more than 60 matK throughout fern lineages, covering 23 families and 55 genera, with novel primers sets aimed at the coding region. Our analyses first demonstrate the extremely high phylogenetic information from fern matK gene (more than 90% are variable sites), compared to rbcL (less than 40%). The inferred tree depicts well-resolved familial relationships with good statistical supports. We therefore recommend the usage of matK to resolve previously unexplored or uncertain phylogenetic relationships in ferns.


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1 - National Taiwan University, Department of Life Science, No. 1, Sec 4, Roosevelt Road, Taipei,, 106, Taiwan, ROC
2 - National Taiwan University, Department of Life Science, No. 1, Sec 4, Roosevelt Road, Taipei, 106, Taiwan, ROC

Keywords:
Fern
matK
phylogeny
barcoding
chloroplast marker.

Presentation Type: Recent Topics Poster
Session: P
Location: Ball Room & Party Room/SUB
Date: Monday, July 28th, 2008
Time: 12:30 PM
Number: PRT017
Abstract ID:1145


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