Recent Topics Posters
Hawkins, Angela , Randle, Christopher P. .
A preliminary examination of subspecific classification within Phoradendron serotinum (Santalaceae): development of microsatellite markers for assessment of population genetic structure.
Phoradendron serotinum, (leafy mistletoe) is a hemi-parasitic plant of the family Santalaceae found in the United States and Mexico. P. serotinum has been divided into four subspecies: subsp. tomentosum, subsp. macrophyllum and subsp. serotinum which occur in the eastern United States from southern New Jersey to southern Florida, through the Midwest south of Oklahoma and into Mexico, and on the west coast from Oregon to Baja, California. Subspecies angustifolium grows in isolated regions of Central Mexico. Subspecies may be difficult to identify based on morphology alone. For instance subspecies serotinum is distinguished from the others by the presence of lightly puberulent leaves (opposed to glabrescent to short pubescent). Just within subspecies serotinum leaves can be elongated, obovate or spathulate (opposed to elliptical, ovate, or orbicular for the other three subspecies). This overlap is especially evident in eastern Texas as these characters that may otherwise be diagnostic of subspecies do not adequately separate three of the subspecies (macrophyllum, tomentosum, and serotinum) that grow in this region. Molecular and morphometric analyses are being utilized to resolve taxonomic confusion within Phroadendron serotinum. Total genomic DNA has been isolated from fresh tissue of more than 100 Phoradendron serotinum samples from three areas in Texas (Huntsville, College Station, and Del Rio). Microsatellite regions have been isolated using a hybridization-capture method. Twelve potential primer sequences have been identified and will be used in future molecular assessments. Additional specimens will be collected from the remainder of species distribution and sampled for genetic differentiation. Ultimately, we hope to provide genetic evidence for classification that will be used in conjunction with morphology.
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1 - Sam Houston State University, Department of Biology, 1900 Avenue I, Huntsville, Texas, 77340, US
2 - Sam Houston State University, Department of Biological Sciences, 1900 Avenue I., Huntsville, TX, 77340
Presentation Type: Recent Topics Poster
Location: Ball Room & Party Room/SUB
Date: Monday, July 28th, 2008
Time: 12:30 PM