Rieseberg, Loren H. .
Population genetic challenges and the potential of modern genomics technologies for the management and characterization of plant genetic resources.
The development of molecular diagnostic tools for the management and characterization of crop germplasm such as landraces, breeder’s varieties, as well as populations of wild relatives is useful for several reasons. An appropriate method could provide a standardized means for identifying and categorizing germplasm across species and across institutions. It could also be used to reduce unwanted duplication in germplasm repositories, assess genetic relationships, develop a more stable classification of domesticated and wild populations, and detect contaminated or admixed samples. Furthermore, if biologically relevant molecular variation were assayed, it might be feasible to predict the likely value of germplasm for breeding and crop improvement. A variety of different approaches are currently being employed to characterize germplasm in different crops, ranging from allozymes to microsatellites to single nucleotide polymorphisms (SNPs) in nuclear loci. Also, DNA-barcoding approaches, including whole plastome sequencing, are now being considered for analyses of clonal and selfing crops. I will explore the strengths and weaknesses of the primary methods currently being employed (or that have recently become technically feasible) for germplasm characterization. I will also discuss the population genetic challenges associated with the development of a widely applicable, stable, and cost-effective strategy for analyzing crops that vary in mating system, ploidy level, and means of propagation. When assessing different approaches, I will do so in the light of rapid advances in sequencing and SNP genotyping technologies that are providing new technological solutions to old problems.
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WS1 Applying Modern Genomic Tools to the Management and Characterization of Plant Genetic Resources
1 - University of British Columbia, Botany Department, 3529-6270 University Boulevard, Vancouver, British Columbia, V6T 1Z4, Canada
Presentation Type: Workshop
Location: Blair CD/Gage
Date: Sunday, July 27th, 2008
Time: 1:00 PM