Dlugosch, Katrina M .
Prospects and challenges of 454 sequencing for PGR characterization.
Roche 454 Life Science GS FLX sequencing is a next-generation technology that currently yields ~100 megabases of sequence per run, in ~250 bp lengths. These are relatively long reads among the next-gen approaches available, and they offer the potential to reconstruct complex (repetitive) nuclear genomic sequence without the use of an existing template for assembly. Where a template is available to aid assembly, long reads also offer the possibility of obtaining large amounts of sequence at low coverage. I will relate some of our own experiences with comparative genomics from low-coverage GS FLX data, and I will detail the laboratory and bioinformatic requirements of using this approach to manage plant genetic resources.
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WS1 Applying Modern Genomic Tools to the Management and Characterization of Plant Genetic Resources
1 - University of British Columbia, Botany, 3529-6270 University Blvd., Vancouver, BC, V6T 1Z4, Canada
Presentation Type: Workshop
Location: Blair CD/Gage
Date: Sunday, July 27th, 2008
Time: 4:00 PM