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Abstract Detail


Molecular Biology

Lebeau, Meagan [1], Paul, Gina [1], Tesheira, Nicola [1], Conrad, Peter L. [1], Marchut Conrad, Janice [1].

Chlamydomonas reinhardtii: The Role of Membrane Bound Hydrogen Pumps In Cadmium Induced Cell Expansion.

Cadmium is a highly toxic heavy metal that has damaging effects on the environment. Its accumulation in soil and aquatic environments is due to many years of improper disposal (Nishikawa et al., 2003). In addition to use in photosynthetic and flagella research, Chlamydomonas research in bioremediation is relevant (Merchant et al., 2007). In the presence of low-intensity white light, cadmium induces cell expansion in C. reinhardtii, compared to white light control (p < 0.001). We show cadmium accumulation, using leadmium fluorescence microscopy, in confirmed viable cells that correlates to cell expansion. We investigated spectral requirement of the cadmium-induced white light response, because dark incubated cells do not respond to cadmium, as well as the involvement of plasma membrane H+- ATPase, vacuolar H+- ATPase, H+-PPase as well as reactive oxygen species in the cadmium induced response.


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1 - State University of New York College at Plattsburgh, Biological Sciences, 101 Broad Street, Plattsburgh, NY, 12901, Clinton

Keywords:
Chlamydomonas
Cadmium
V-H+-PPase
hydrogen pump
bioremediation.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Ball Room & Party Room/SUB
Date: Monday, July 28th, 2008
Time: 12:30 PM
Number: PMB001
Abstract ID:149


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