Harbaugh, Danica T. , Wagner, Warren L. , Percy, Diana , James, Helen F. , Fleischer, Robert C. .
Genetic structure of the polymorphic Metrosideros (Myrtaceae) complex in the Hawaiian Islands using nuclear microsatellites.
Hawaiian Metrosideros polymorpha (Myrtaceae) and related taxa form a morphologically diverse complex that dominates the landscape, occupying a wide variety of habitats from sea-level to over 8,500 feet in elevation. To uncover the genetic structure of Hawaiian Metrosideros, 10 nuclear microsatellite loci were genotyped using published primers for 150 individuals from across all of the major Hawaiian Islands. These data were used to test two competing hypotheses that arose from analysis of chloroplast DNA sequence data showing a predominant geographic structure of island clades: are plants from particular islands/locations, or are plants from the same taxon/morphology more genetically similar? In addition to estimating the amount of gene flow and heterozygosity, the genetic structure of the microsatellite data was estimated using AMOVA, distance analysis, and the Bayesian algorithm of the computer program STRUCTURE. Results demonstrate that Hawaiian Metrosideros is a dynamic complex, with data supporting instances of dispersal between islands, as well as parallel evolution within islands, indicating that the current taxonomy may not reflect true lineages. In addition, the data support limited chloroplast capture when compared with the previously published analysis of chloroplast sequence data.
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1 - National Museum of Natural History, Smithsonian Institution, Botany, P.O. Box 37012, MRC 166, Washington, DC, 20013-7012, USA
2 - 6338 Hawthorn Lane, Vancouver, British Columbia, V6T 2J6, Canada
3 - National Museum of Natural History, Smithsonian Institution, Division of Birds, PO Box 37012, MRC 116, Washington, DC, 20013-7012, USA
4 - National Zoological Park, Smithsonian Institution, Center for Conservation and Evolutionary Genetics, 3001 Connecticut Ave., NW, Washington, DC, 20008-0551, USA
Presentation Type: Oral Paper:Papers for Topics
Date: Tuesday, July 29th, 2008
Time: 8:15 AM