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Abstract Detail


Genomics / Proteomics

Shah, Azad H. [1], Wimmer, Monika A. [2].

Boron-binding proteins from wheat.

Boron (B) is an essential micronutrient for plants but minor increase in its concentration, especially in conjunction with salinity causes severe stress for plants which determines yield (Wimmer et al. 2003). In cell wall, B can cross-link pectic polysaccharides through borate–diol bonding of two rhamnogalacturonan II (RG-II) molecules (O’Neill et al., 2004). The aim of this proteomics based project was to isolate and characterize boron binding proteins from cell sap of wheat (Triticum aestivum cv Thasos) leaves using boronic acid affinity column chromatography. Hydroponic culture was used to grow wheat plants at two levels of B toxicity (200 μM and 800 μM). Cell sap was extracted from leaves using standard protocols and subject to protein purification using agarose bound phenyl boronic acid. Putative boron binding proteins interact with boronic acid and are considered candidates for possible B function. Proteomics based approach was used to compare the retarded and non-retarded protein fractions using 2-D gel electrophoresis.
Comparison of treatments with control revealed several candidate proteins showing a strong binding affinity for boron. The results indicate that along with other proteins Rubisco may also be involved in plant defence mechanism against B toxicity. A further investigation on identification of these putative boron binding proteins is underway using mass spectrometery.


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1 - University of the Punjab, School of Biologcial Sciences, Quaid-e-Azam Campus (New Campus), Lahore, Punjab, 54590, Pakistan
2 - University of Bonn, Institute of Crop Science and Resource Conservation,, Karlrobert-Kreiten-Str. 13, Bonn, D-53115, Germany

Keywords:
Boron-binding
Proteins
wheat
Affinity-chromatography.

Presentation Type: Oral Paper:Papers for Topics
Session: 18
Location: 182/I K Barber
Date: Monday, July 28th, 2008
Time: 1:30 PM
Number: 18001
Abstract ID:177


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