Systematics/Phytogeography / Taxonomie/ Section
Neubig, Kurt , Whitten, W. M. , Carlsward, Barbara S. , Blanco, Mario A. , Endara, Lorena , Williams, N. H. , Moore, Michael .
Phylogenetic utility of ycf1 in Orchids: an underappreciated plastid DNA region.
Plastid DNA sequences have been favored by systematists for reconstructing phylogenies of plant groups because of their variability and ease of amplification. The utility of any DNA region for phylogenetic analysis is determined by ease of amplification and alignment, and variability across broad taxon sampling. Often, a compromise must be made between using relatively highly conserved coding regions and highly variable introns or intergenic spacers. Analyses of a combination of these types of DNA regions yield phylogenetic structure at various levels of a tree (i.e., along the spine and at the tips of the branches). Here we demonstrate the phylogenetic utility of a heretofore unused portion of the plastid protein-coding gene ycf1 in orchids. All portions of ycf1 examined are highly variable, yet alignable across Orchidaceae, and are phylogenetically informative at species level. In Orchidaceae, ycf1 is more variable than matK both in total number of parsimony informative characters and percent variability. ITS (nrDNA) is more variable than ycf1 but is more difficult to align. Although we only demonstrate the phylogenetic utility of ycf1 only in orchids, it is likely to be similarly useful among other plant taxa.
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1 - University of Florida, Department of Botany, 220 Bartram Hall, P.O. Box 118526, Gainesville, Florida, 32611, USA
2 - University of Florida, Florida Museum of Natural History, Gainesville, Florida, 32611-7800, USA
3 - Eastern Illinois University, Department of Biological Sciences, 600 Lincoln Avenue, Charleston, IL, 61920, USA
4 - University of Florida, Department of Botany, 220 Bartram Hall, P.O. Box 118526, Gainesville, Florida, 32611-8526, USA
5 - Oberlin College, Biology Department, 119 Woodland Street, Science Center K111, Oberlin, Ohio, 44074-1097, USA
Presentation Type: Poster:Posters for Sections
Location: Ball Room & Party Room/SUB
Date: Monday, July 28th, 2008
Time: 12:30 PM