Li, Fay-Wei , Kuo, Li-Yaung , Chiou, Wen-Liang , Wang, Chun-Neng .
Tissue-Direct PCR, a rapid and extraction-free method for fern barcoding.
DNA extraction has always been the most laborious and time-consuming step for DNA barcoding. Here we have developed an efficient procedure called “Tissue-Direct PCR”, in which a slice of untreated fern tissue is mixed with PCR reagents allowing certain genomic regions to be amplified in thermal cyclers directly. Gametophytes and young sporophytes often provide little material for DNA extraction and are particularly difficult to be identified morphologically. For these developmental stages of ferns, Tissue-Direct PCR provides promising results achieving more than 80% successful rates on amplifying several potential barcoding regions (rbcL, trnH-psbA and trnL-F). Therefore, in combining Tissue-Direct PCR with barcoding system, identification for immature ferns could become very easy and rapid. Tissue-Direct PCR would also be very helpful on large scale studies such as surveying fern gametophytes’ ecological distributions and population structures.
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1 - National Taiwan University, Department of Life Science, No. 1, Sec 4, Roosevelt Road, Taipei, 106, Taiwan, ROC
2 - National Taiwan University, Department of Life Science, No. 1, Sec 4, Roosevelt Road, Taipei, 106, Taiwan
3 - Taiwan Forestry Research Institute, 53 Nan-Hai Rd, Taipei, 100, Taiwan
Presentation Type: Poster:Posters for Sections
Location: Ball Room & Party Room/SUB
Date: Monday, July 28th, 2008
Time: 12:30 PM