Das, Songhita , Chi Lun, Pui , Mehdy, Mona .
Understanding the Mechanisms and Functions of French Bean PvPRP1 Gene Down-regulation During the Defense Response in Transgenic Arabidopsis.
During the defense response to pathogen attack and wounding, plant cells modify their walls to produce an effective barrier to pathogen invasion. This response involves both up-regulation and down-regulation of cell wall protein genes. One such down-regulated gene is the French bean proline-rich protein, PvPRP1. The PvPRP1 mRNA exhibits an elicitor-induced reduction in its half-life. The PvPRP1 protein has less cross linking potential due to low tyrosine content compared to the other tyrosine-rich proline-rich proteins, and is hypothesized not to contribute to cell wall strengthening. A 50-kD protein designated PRP-BP was found to bind specifically with the 3-UTR of the PvPRP1 mRNA in cellular extracts and is hypothesized to contribute to PvPRP1 mRNA down-regulation. The 3-UTR (917-1111 bases) has two AUUUA motifs (940 -967 bases and 1042-1046 bases). In many mRNAs, existence of specific cis elements like the DST sequences or the AU-rich elements ( AREs) in their 3UTR region seem to be a common feature affecting mRNA stability and regulation of mRNA half life. These elements on the mRNA interact with the trans acting proteins factors or some miRNAs that bind to it post-transcriptionally. In our case, PRP-BP binds to a region containing the first AUUUA in vitro. In this study, different PvPRP1 constructs were transformed into the Arabidopsis system. The constructs have either (1) the full-length 3-UTR; (2) a truncated 3-UTR containing only the first AUUUA motif; (3) a truncated 3-UTR without AUUUA motifs. We will present the analysis of the PvPRP1 mRNAs in transgenic Arabidopsis plants treated with different defense-inducing compounds. Establishment of this system in Arabidopsis will enable elucidation of the signaling pathway through mutant screens.
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1 - University of Texas, Austin, Section of Molecular, Cellular and Developmental biology, Institute of cellular and Molecular Biology, 1 University station, A6720, Biology Building, 3rd floor MCDB office, Austin, texas, 78731, USA
2 - University of Texas, Austin, Section of Biology, 1 University station, A 6720, Austin, Texas, 78731, USA
3 - University of Texas, Austin, Section of Molecular, Cellular and Developmental Biology and Institute for Cellular and Molecular Biology,, Section of Molecular Cell and Developmental Biology, College of Natural Sciences, 1 University station, A6700, Austin, Texas, 78712, USa
Presentation Type: Poster:Posters for Topics
Location: Ball Room & Party Room/SUB
Date: Monday, July 28th, 2008
Time: 12:30 PM