Oliveira, Luiz Orlando , Rossi, Ana A. Bandini , Venturini, Bruna Afonso , Silva, Roberta dos Santos , Garcia, Magali Gonçalves , Batista, Flávia Reis de Carvalho .
Molecular phylogeography of ipecac (Psychotria ipecacuanha).
Ipecac (Psychotria ipecacuanha - Rubiaceae) is a medicinal species that occupied a prominent post within the traditional medicine of native Brazilians. In recent times, careless exploitation and human-led deforestation led to a severe demographic decline of wild ipecacs. The contemporary populations occur in three clearly-defined ranges: 1- Central America and northern parts of South America; 2- Southeastern part of the Brazilian Amazon; and 3- Atlantic Rain Forest along the Brazilian coast. We used sequence data from the plastid and nuclear genomes to carry out a molecular phylogeographic study of ipecac in the entire species distribution. Sequencing of the trnT-trnL region of the chloroplast genome distinguished 13 haplotypes among 120 individuals from 25 populations. While the Amazonian and Central American ranges displayed a single haplotype each, the Atlantic range showed eleven haplotypes. None of the haplotypes are shared among ranges. A single haplotype network was obtained, with haplotypes of Atlantic origin occupying a central position and connecting the Amazonian haplotype and the Central American haplotype. Sequences obtained from the internal transcribed spacer (ITS) of the nuclear genome corroborate with cpDNA results to reveal the Atlantic populations as having higher genetic diversity. Many individuals of the Atlantic range presented intraindividual ITS variation, while single ribotypes were present in the Amazonian and Central American ranges. The molecular data favors the Atlantic range as harboring ancestral populations from which populations of the other two ranges might have derived.
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1 - Federal University of Viçosa, Instituto de Biotecnologia Aplicada à Agropecuária, Viçosa, MG, 36570-000, Brazil
2 - Universidade do Estado de Mato Grosso, Departamento de Ciências Biológicas, Alta Floresta, MT, 78580-000, Brazil
3 - Universidade Federal de Viçosa, Instituto de Biotecnologia Aplicada à Agropecuaria, Viçosa, MG, 36570-000, Brazil
4 - Universidade Federal de Viçosa, Instituto de Biotecnologia Aplicada à Agropecuaria, Viçosa, MG, 36570-000, Brazil
5 - Universidade Federal de Viçosa, Instituto de Biotecnologia Aplicada à Agropecuaria,, Viçosa, MG, 36570-000, Brazil
population genetic structure
tropical dry forest
Presentation Type: Oral Paper:Papers for Topics
Date: Monday, July 28th, 2008
Time: 3:30 PM