Evolutionary Developmental Biology (Evo-Devo)
Bruggeman, Clare , Gleissberg, Stefan .
Investigating the effects of phytohormones on leaf dissection in the California Poppy, Eschscholzia californica.
Leaves are determinate lateral organs that pass through a sequence of phases: initiation, primary morphogenesis, tissue differentiation, and finally maturation. Primary morphogenesis is pronounced in dissected-leaf plants because of the initiation of leaflets along the primordial leaf margins. Members of the KNOX family of transcription factors, among others, are implicated in the process of leaf dissection in some species. In Arabidopsis, KNOX genes are known to be necessary for proper shoot apical meristem development, and exert this function through interaction with various phytohormones. The extent to which this regulatory module was co-opted in various lineages to control leaf dissection remains largely unknown. Eschscholzia californica is an emerging model plant that can serve as a reference system for the basal eudicot lineage Ranunculales for evolutionary developmental studies. Here we report on ongoing investigations of the role of phytohormones in primary morphogenesis at the leaf margins of Eschscholzia. In a tissue culture system, Eschscholzia seedlings are subjected to a range of treatments including NPA (an auxin transport inhibitor), gibberellin (GAs), and cytokinin (CKs). Effects on leaf dissection are studied using time-lapse photography and scanning electron microscopy. Preliminary data indicates an involvement of all three phytohormones in the regulation of leaf dissection in this basal eudicot species. To evaluate correlations between phytohormones and Eschscholzia homologs of class1 KNOX genes, semi-quantitative RT-PCR is being used.
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1 - Ohio University, Department of Enivronmental and Plant Biology, 315 Porter Hall, Ohio University, Athens, OH, 45701, USA
2 - Ohio University, Environmental and Plant Biology, Porter Hall, Athens, Ohio, 45701, USA
Presentation Type: Poster:Posters for Topics
Location: Ball Room & Party Room/SUB
Date: Monday, July 28th, 2008
Time: 12:30 PM