| Abstract Detail
Polyploidy: Genetics, Evolution and Ecology Coate, Jeremy E. [1], Ilut, Daniel [2], May, Greg [3], Farmer, Andrew [3], Doyle, Jeff J. [2]. The evolution of photosynthesis in Glycine allopolyploids. The perennial Glycine allopolyploid complex comprises eight tetraploid species formed by various combinations of eight diploid genomes. All of the allopolyploids appear to have originated recently, most of them multiple times, and some of them with both diploid progenitors as chloroplast genome donors. Several of the polyploids have expanded their ranges to islands of the Pacific Ocean, whereas all diploid perennial Glycine species are virtually confined to Australia. An ultimate research goal is to understand the basis for the apparently greater "success" of these polyploids. Proximal goals include identifying physiological changes correlating with allopolyploidy and characterizing them at the molecular level. By comparing the different Glycine allopolyploids we hope to identify emergent properties of allopolyploidy in the group. We have identified differences in photosynthetic capacity and photoprotection between Glycine allopolyploids and their diploid progenitors. One polyploid species (G. tomentella T2) has a higher photosynthetic capacity than its two progenitors, and also has a greater capacity for photoprotection under excess light conditions, exhibiting less irreversible damage to Photosystem II. We are using Solexa (Illumina) technology for high-throughput sequencing of transcriptomes of the polyploid and its progenitors under the same conditions in which chlorophyll fluorescence measurements were conducted, to determine whether genes involved in photosynthesis and photoprotection show dosage effects from polyploidy, and to determine the relative contributions of homoeologous genes to expression in the polyploid.
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1 - Cornell University, Plant Biology, 228 Plant Science Building, Ithaca, NY, 14853, USA
2 - Cornell University, L.H. Bailey Hortorium, Department of Plant Biology, Ithaca, New York, 14853, USA
3 - National Center for Genome Resources
Keywords:
Allopolyploidy
Glycine
Photosynthesis
transcriptome
gene expression evolution.
Presentation Type: Symposium or Colloquium Presentation
Session: S8
Location: Room 2/Woodward
Date: Tuesday, July 29th, 2008
Time: 4:30 PM
Number: S8007
Abstract ID:353 |
