Dlugosch, Katrina M , Barker, Michael S. , Lai, Zhao , Rieseberg, Loren H. .
Ecological genomics of species invasions: understanding hybridization and adaptation in yellow starthistle (Centaurea solstitialis) through expressed sequence variation.
Comparative genomic techniques have the potential to give us novel and particularly precise insights into the traits that facilitate the establishment and local adaptation of introduced species. We are applying genomic tools to the study of yellow starthistle (Centaurea solstitialis, Compositae), a noxious invader of grasslands in Mediterranean climates. To understand which alleles have been favoured by novel selective regimes in starthistle’s introduced range, we are comparing transcribed sequences among native and invading individuals using both traditional sequencing and Roche / 454 Life Sciences GS FLX next-generation sequencing of EST's. We report on several methodological issues: the editing and assembly of 454 cDNA sequence reads, the comparison of variation among individuals with incomplete genomic data, and the identification of appropriate native/invading population comparisons. We present our findings of loci under selection and cryptic hybridization in yellow starthistle invasions. We also describe a new open-source software for reference-guided next-generation sequence assembly: SCARF (SCaffolded Assembly of Roche 454).
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1 - University of British Columbia, Botany, 3529-6270 University Blvd., Vancouver, BC, V6T 1Z4, Canada
2 - Indiana University, Department of Biology, Jordan Hall, 1001 East Third Street, Bloomington, Indiana, 47405, USA
3 - Indiana University, Department of Biology and Center for Genomics and Bioinformatics, 1001 East Third St., Bloomington, IN, 47405, USA
4 - University of British Columbia, Botany Department, 3529-6270 University Boulevard, Vancouver, British Columbia, V6T 1Z4, Canada
Presentation Type: Oral Paper:Papers for Sections
Location: 182/I K Barber
Date: Monday, July 28th, 2008
Time: 10:00 AM