Molecular Ecology and Evolution
Plasencia López, Lucía María Teresa , Mandujano, Maria .
Clonal diversity and optimum crosses in Opuntia rastrera Weber (Cactaceae).
Species of genus Opuntia present clonal propagation. As a consequence, local clonal spatial distribution can produce genetic related patches, an attribute that can have a strong influence over the population mating system. The objectives of this study were: 1) to determine the clonal diversity and distribution of two populations of Opuntia rastrera with two types of recruitment (sexual and clonal). 2) check for the influence of the clonality in small distances using demographic information and, 3) to determine the optimum cross distance, measuring the effect that genetic similitude has over the reproductive fitness. With the Inter Simple Sequence Repeated amplification (ISSR’s), we estimated: the clonal diversity in both populations and the genetic similitude between pairs of parents. With the known identities we built distribution clonal maps for both populations. Field experiments consisted in crossing experiments with pollen addition using three distances between pollen receptive plants and pollen donator. We found that clonal site is as diverse as the sexual site. Most individuals have one or two copies and only few have more added copies. There is a high genetic flow between both populations. In the optimum crossing distance experiment, plants separated by >50m of distance showed a higher production of fruits and seeds than plants at <10m. Genetic similarity decreased with increasing distance between plants. Fruit and seed production increased as genetic similarity between pairs of parent plants decreases.
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Version pdf de tesis UNAM
1 - Universidad Nacional Autonoma de Mexico, Instituto de Ecologia, Ciudad Universitaria, A.P. 70-275, Mexico, D.F., 04510, Mexico
Presentation Type: Poster:Posters for Topics
Location: Ball Room & Party Room/SUB
Date: Monday, July 28th, 2008
Time: 12:30 PM