Ruck, Elizabeth , Ashworth, Matt P. , Nakov, Teofil , Zhengqiu, Cai , Theriot, Edward , Jansen, Robert K. , Andersen, Robert , Julius, Mathew , Draisma, Stefano , Kawai, Hiroshi .
Assessing multi-gene candidates for an algal heterokont phylogeny.
Heterokonts (or stramenopiles) are a group of protists with unequal flagella that include both algal and colorless representatives. The algal heterokonts are currently divided into 16 classes and include over 100,000 described species. Common members include the brown seaweeds, chrysophytes and diatoms. Despite 200 years of light microscopic study, 50 years of electron microscopic study, and 20 years of molecular investigations, phylogenetic relationships among the 16 classes remains unclear. As part of a Tree of Life (ATOL) grant we plan to estimate relationships among these classes by generating a large multi-gene molecular data set. The past year has been devoted to growing cultures and performing a pilot study of 27 taxa. The purpose of the pilot study is to assess candidate molecular markers before a final selection of seven genes (representing the nuclear, mitochondrial and plastid genomes) for the expanded taxon sampling of 270 algal heterokont species and 30 non-photosynthetic relatives. Our preliminary studies include the nuclear genes SSU rRNA, actin, alpha and beta tubulin, the plastid genes rbcL and psbC, and the mitochondrial genes cox1 and cob. To date, we’ve assessed and decided to move forward with 1 nuclear (SSU rDNA) and two chloroplast (rbcL and psbC,) genes and are awaiting further data before choosing the remaining 4 molecular markers.
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1 - University of Texas at Austin, Section of Integrative Biology, Austin, TX, 78712, USA
2 - University of Texas at Austin, Texas Natural Science Center, 2400 Trinity Street, Austin, TX, 78705, USA
3 - University of Texas at Austin, Section of Integrative Biology, 1 University Station, #A6700, Austin, TX, 78712, USA
4 - Bigelow Laboratory for Ocean Sciences, P.O. Box 475, 180 McKown Point Road, West Boothbay Harbor, Maine, 04575-0475, USA
5 - St. Cloud State University, Department of Biological Sciences, 720 4th Avenue S, St. Cloud, MN, 56301, USA
6 - Nationaal Herbarium Nederland, Leiden University branch (NHN-L), P.O. Box 9514, Leiden, The Netherlands
7 - Kobe University Research Center for Inland Seas (KURCIS)?Kobe Universi, 1-1 Rokkodai, Nadaku, Kobe, 657-8501, Japan
Presentation Type: Oral Paper:Papers for Sections
Date: Monday, July 28th, 2008
Time: 11:15 AM