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Abstract Detail


Plant Development / Développement des plantes (CBA/ABC)

Sawhney, Vipen, K. [1], Sheoran, Inder, S. [1].

Proteomics of tomato seed and pollen.

Seed plants produce two dispersal structures, the seed and pollen and the former is for the dispersal of embryo and the latter for sperm cells. Both these structures are highly desiccated and have protective structures, the seed coat and pollen wall respectively, which provide protection to inner structures from desiccation and pathogen attack. We have used the proteomic approach to identify the proteins present in these two structures and to determine their potential roles in the mature structures and for subsequent germination and growth. Proteins were extracted from mature tomato seed and pollen, separated by 1- and 2-D gel electrophoresis, analyzed by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS), and identified by searching the NCBInr and EST databases.
The major proteins in both the seed and pollen were defense-related which could be part of the survival strategy of these small independent structures. The tomato seed, like many other angiosperm seed, had a number of storage proteins, e.g., vicilins, albumins, prolamins and legumins which would potentially be required during the germination process. In contrast, storage proteins were not detected in tomato pollen but a number of proteins related with desiccation tolerance, e.g., the LEA proteins, were present in both structures. In addition, cytoskeleton–related proteins, proteins involved in Ca2+ signaling, glycine-rich proteins, proteins involved with nucleic acid metabolism and general metabolism, were identified which have potential roles in germination and growth of the seed and pollen.


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1 - University of Saskatchewan, Biology, 112 Science Place, Saskatoon, Sask, S7N 5E2, Canada

Keywords:
proteomics
Seed
pollen
tomato
seed germination.

Presentation Type: Oral Paper:Papers for Sections
Session: 19
Location: 215/SUB
Date: Monday, July 28th, 2008
Time: 2:15 PM
Number: 19004
Abstract ID:448


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