Systematics/Phytogeography / Taxonomie/ Section
Choi, Hong-Keun , Shin, Hyunchur , Chang, Yung-ta , Kim, Changkyun .
Genetic diversity and relationship of diploid and polyploid species of Isoetes in East Asia based on amplified fragment length polymorphism (AFLP) analysis.
Isoetes species are critically endangered aquatic¬†vascular plants¬†in East Asia. We aimed to evaluate the levels of genetic diversity and genetic relationship among diploid, tetraploid, and hexaploid Isoetes species of East Asia using amplified fragment length polymorphism (AFLP). The combinations of three AFLP selective primers generate a total of 923 amplification products of which 919 bands(99.6%) are detected as having polymorphisms. The levels of genetic diversity in two diploid species (I. taiwanensis [P = 33.1% - 38.3%] and I. asiatica [P = 49.0%]) are higher than in polyploid species (tetraploid, I. sp1 [P = 9.3% - 29.3%] and I. sp2 [P = 22.3%]; hexaploid, I. coreana [P = 1.6% - 20.6%] and I. japonica [P = 5.6% - 20.5%]). However, the level of genetic differentiation in the populations of I. coreana (P = 43.5%) are similar to I. taiwanensis (P = 47.1%), suggesting the polyploid species indeed have higher levels of selfing than the diploid species. A dendrogram drawn by neighbor joining analysis shows three clusters; one that represents I. asiatica, the other one I. japonica, and the rest species cluster that are divided into two subclusters (the first subcluster of I. taiwanensis and I. sp1, and the second one of I. coreana and I. sp2). Furthermore, polyploid Isoetes species are not grouped into same clusters. The principal coordinate analysis is congruent with the result of NJ analysis. As a result of our study for six Isoetes species in East Asia, the genetic diversity of each species is very variable regardless of their distribution. Also the results suggest that each species has been isolated to their habitats through polyploidy formation independently.
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1 - Soonchunhyang University, Department of Biology, Asan, 336-745, Republic of Korea
2 - National Taiwan Normal University, Department of Life Science, Taipei, Taiwan
3 - Ajou University, Biological Sciences, Suwon, 443-749, Republic of Korea
Presentation Type: Poster:Posters for Sections
Location: Ball Room & Party Room/SUB
Date: Monday, July 28th, 2008
Time: 12:30 PM