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Abstract Detail


Pteridological Section/AFS

Schuettpelz, Eric [1], Grusz, Amanda L. [1], Windham, Michael [1], Pryer, Kathleen M. [1].

The utility of nuclear gapCp in resolving polyploid fern origins.

Although polyploidy is rampant in ferns and plays a major role in shaping their diversity, the evolutionary history of most polyploid taxa remains unclear. Isozyme electrophoresis can provide important clues regarding polyploid origins; however, low-copy nuclear gene sequencing holds even greater potential as it can be used to place polyploid genomes in a phylogenetic context. Here, we report the development of new primers for the amplification of a portion of the nuclear gapCp gene. Through a broad survey across ferns, we demonstrate that these new primers are nearly universal for this clade. With a case study in cheilanthoids, we show that this rapidly evolving marker is a powerful tool for uncovering polyploid ancestries. Our results indicate that gapCp holds considerable potential for addressing species-level questions across the fern tree of life.


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1 - Duke University, Department of Biology, Durham, NC, 27708, USA

Keywords:
ferns
gapCp
GAPDH
low-copy nuclear gene
molecular systematics
nuclear marker
polyploidy
Pteridaceae
species-level phylogenetics.

Presentation Type: Oral Paper:Papers for Sections
Session: 23
Location: 209/SUB
Date: Monday, July 28th, 2008
Time: 3:00 PM
Number: 23005
Abstract ID:514


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