Gene Structure and Function
Grover, Monendra , Maheshwari, S.C. , Tyagi, A.K. .
Signal transduction events in chloroplast gene expression in rice.
Though the role of calcium in plant and animal systems is well documeneted , much remains to be done to analyze its effect on expression of chloroplast-encoded genes. To conclusively analyze any role of calcium in chloroplast gene expression, various strategies were used. The treatment of seedlings with exogenous calcium resulted in increase in gene expression in dark, i.e. in the etioplast system. Application of ionophore also resulted in increase in gene expression, possibly by promoting entry of calcium. Treatment with EGTA, a chelator of calcium ions, and with calcium ion channel blockers such as verapamil resulted in decrease in gene expression in light. Thus calcium seems to play an important role in regulating light dependent activation of chloroplast genes. The present work in rice also reveals that cGMP levels may affect the accumulation of psbA, psaA and rbcL mRNA. The role of protein phosphatases was demonstrated in the chloroplast gene expression in rice by using protein phosphatase inhibitors.
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1 - Amity University, Amity Institute of Biotechnology, Sector 125 Expressway, Noida, Uttar Pradesh, India
2 - University of Delhi South Campus, Department of Plant Molecular Biology, Benito Juarez Road, New Delhi, New Delhi, India
Presentation Type: Oral Paper:Papers for Topics
Date: Monday, July 28th, 2008
Time: 2:45 PM