Craft, KJ , Wenger, Jonathan P , Weiblen, George D .
Segregation distortion and genetic mapping of cannabinoids in an inbred population of Cannabis sativa.
We screened an inbred population of Cannabis sativa at 16 polymorphic microsatellite loci for the purpose of quantitative trait locus mapping in this economically important species. Dioecious hemp and marijuana cultivars were subject to five generations of full-sib inbreeding and then crossed to produce an F1 mapping population. A single F1 was selfed using silver nitrate to induce fertile male flowers in a genetically female plant. Sixteen microsatellite loci that were heterozygous in the F1 were screened in 136 F2 plants. Two of the 16 loci showed significant departure from Mendelian expectation, which we attribute to segregation distortion. Based on tests of genotypic linkage disequilibrium, we have identified 8 linkage groups, one of which segregates with cannabinoid content. The difference in relative abundance of tetrahydrocannabinol (THC) and cannabidiol (CBD) between marijuana and hemp can be mapped quantitative genetically.
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1 - University of Minnesota, Plant Biology, 250 Biological Sciences Center, 1445 Gortner Ave., St. Paul, MN, 55108, U.S.A.
2 - University of Minnesota, Department of Plant Biology, 250 Biological Sciences Center, 1445 Gortner Avenue, Saint Paul, MN, 55108, USA
3 - University of Minnesota, Department of Plant Biology, 250 Biological Sciences, 1445 Gortner Avenue, St. Paul, Minnesota, 55108, USA
Presentation Type: Oral Paper:Papers for Sections
Location: 182/I K Barber
Date: Monday, July 28th, 2008
Time: 10:30 AM