Evolutionary Developmental Biology (Evo-Devo)
Ojeda, Isidro , Santos-Guerra, Arnoldo , Oliva-Tejera, Felicia , Jaén-Molina, Ruth , Cronk, Quentin .
Barcoding plant island radiation, what works and what does not. A case study in Lotus (Leguminosae) in the Macaronesia.
DNA barcoding is a system for species recognition and identification through the use of standard organellar regions. To date there is no consensus about the most suitable region in plants. However, it is widely assumed that a combination of a two or more plastid gene or spacer regions will be required. A good test of DNA barcoding is whether the technique can differentiate recently diverged species, such as those resulting from an evolutionary radiation. As part of a wider study on the evolution of island plants we tested four chloroplast regions that have been suggested as plant DNA barcoding, trnH-psbA. matK, rpoC1 and rpoB, in a recent radiation of legumes in the genus Lotus (Loteae) from the Macaronesian region (Azores, Canary Islands, Selvage, Madeira and the Cape Verde Islands). This group is divided into two subgenera, Pedrosia and Rhyncholotus, and comprises about 34 species. At least 75 % of the species are endemic, rare or endangered in this archipelago and correct species identification is important in this group for conservation purposes. The two subgenera are easily recognized and differentiated using flower morphology, but within each group, vegetative features are more informative for species identification. In this analysis we included 43 samples representing 22 species which encompasses all lineages that have been identified on these two subgenera. Among the genes included, trnH-psbA and matk had the highest levels of variation and were the most useful for species identification. Species identification was only possible and accurate within the two African and the Cape Verde lineages, and not possible within the most recent radiation of the Canary Islands, where most of the diversity and endemism occur. Although these gene regions are useful for barcoding continental taxa a different approach will be required to barcode island radiations
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1 - University of British Columbia, Botanical Garden and Centre for Plant Research. Department of Botany, 6804 SW Marine Drive, Vancouver, British Columbia, V6T 1Z4, Canada
2 - Jardín de Aclimatación de La Orotava, Puerto de la Cruz, Canary Islands, Tenerife, Spain
3 - Jardín Botánico Canario \"Viera y Clavijo\", Molecular Biodiversity Labs and DNA Bank, Ap. de correos 14 de Tafira Alta., Las Palmas de Gran Canaria, 35017, Spain
4 - Jardín Botánico Canario \"Viera y Clavijo\", Biodiversidad Molecular y Banco de ADN, Ap. de correos 14 de Tafira Alta, Las Palmas de Gran Canaria, 35017, Spain
5 - University of British Columbia, Botany, Centre for Plant Research, MacMillian Building, 2357 Main Mall, Vancouver, BC, V6T 1Z4, Canada
Presentation Type: Poster:Posters for Topics
Location: Ball Room & Party Room/SUB
Date: Monday, July 28th, 2008
Time: 12:30 PM