Erickson, David , Bermingham, Eldredge , Sanjur, Oris , Kress, John .
A two-locus DNA Barcode for plants: species discrimination and community phylogenetics in a tropical ecosystem.
We present data that demonstrate the practical application of a two-locus plant DNA barcode to a tropical community, the 50-ha Forest Dynamics Plot on Barro Colorado Island, Panama. Using a 540 bp segment of the plastid coding locus rbcL and the complete sequence of the intergenic spacer trnH-psbA, we are able to correctly identify all 350 species in the plot and construct a community phylogeny based on a combined locus supermatrix. We use BLAST to demonstrate the resolution of the two-locus barcode in species discrimination and show how this can aid in ecological studies of herbivory and plant animal interactions. Further, we show that when an intergenic spacer marker is employed in combination with a coding locus, use of a supermatrix or Matrix Representation with Parsimony (MRP) accurately reconstruct phylogenetic relationships among all species for a global community phylogeny. Such phylogenies will enable investigation of functional trait evolution and community assembly parameters. Lastly, we directly compare our results with other published studies that advocate alternative plant DNA barcode loci.
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1 - Smithsonian Institution, Department of Botany, National Museum of Natural History, Washington, DC, 20013
2 - Smithsonian Institution, Smithsonian Tropical Research Institute, Balboa, Ancón, Panama
3 - Smithsonian, Botany, PO Box 37012, Washington, D.C, 20013-7012, USA
Matrix Representation with Parsimony.
Presentation Type: Oral Paper:Papers for Topics
Date: Monday, July 28th, 2008
Time: 4:00 PM