Saadatmand, Sara , Fahimy, Hamid , alaediny, alireza .
The study of the physiological effects of lead pollution on the growth parameters and protein content in Medicago sativa L.
In this investigation, the physiological effects of the different concentrations of Pb(No3)2 ( 0.25 – 0.5 – 1 – 2 mM ) on the growth parameters in Medicago sativa L.were studied . After 20 days, the dry and fresh weight of the roots and shoots and its lenght, leaves area, microscopical observation, accumulation of lead in roots and shoots, soluble and insoluble sugars, total protein content and profile of proteins by SDS-PAGE, measured and studied. The results showed that lead toxicity decreased significantly the length of roots and shoots , leaves area ,dry and fresh weight. In the high concentration of Pb+2 , lignin biosynthesis and fiber layer were increased. the lead content in the roots and shoots goes up by increasing level of lead in lead treatments. When the concentration of lead increased, the amount of soluble sugars in shoots and roots were decreased whereas the insoluble sugars in shoots increased and in roots decreased. By increasing lead in treatment in shoots and roots, the total protein goes up. The profile of proteins by SDS-PAGE showed some changes as compared with control plants. This research indicated that the Medicago sativa L. is a heavy metal tolerant plant and absorbe high level of lead from soil and accumulated in roots and shoots tissues.
Log in to add this item to your schedule
1 - Islamic Azad University,Science and research branch, botany, Islamic Azad University,Science and research branch, Tehran, Iran
2 - Botany, Islamic Azad University,science and research branch, Tehran, Iran
Presentation Type: Poster:Posters for Sections
Location: Ball Room & Party Room/SUB
Date: Monday, July 28th, 2008
Time: 12:30 PM