Coleman, Martha , Wieczorek, Ania M. .
Developing a molecular diagnostic tool for invasive alien species utilizing a RAPD-SCAR approach in Leptostemonum (Dunal) Bitter (Solanum L.; Solanaceae).
It has been estimated that control efforts in addition to losses and damages incurred from non-native pests cost the United States nearly US$ 137 billion annually. One of the most severely affected areas of the United States’ economy by non-native pests is the agricultural industry. Although small compared to the continental US, agriculture in Hawaii provides nearly 42, 000 jobs and generates $2.9 billion to the states annual economy. Providing a ‘world-class’ destination for tourists and a diverse agricultural industry makes the state exceptionally vulnerable to invasions by non-native plant pests. A molecular method of species differentiation is essential to the state, as many taxa arrive at ports of entry unidentifiable due to the absence of fruit and flowers. This study offers a rapid, accurate diagnostic tool to be utilized by the Hawaii Department of Agriculture to distinguish between known non-native plant pests and their most closely related relatives. A RAPD-SCAR approach produces unique banding patterns, or ‘fingerprints’, that provide straightforward reproducible results that can be easily analyzed on an electrophoretic gel. This technique allows for the differentiation between Solanum species currently listed on Hawaii’s noxious weed list, and the further development of species specific primers. Future application on a national, and perhaps even global scale is expected; especially in US territories such as Guam and in countries such as Australia and New Zealand where invasive species prevention is of great importance.
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1 - University of Hawaii at Manoa, Department of Tropical Plant and Soil Sciences, 3190 Maile Way, St. John 102, Honolulu, HI, 96822, US
Presentation Type: Poster:Posters for Sections
Location: Ball Room & Party Room/SUB
Date: Monday, July 28th, 2008
Time: 12:30 PM