Gene Structure and Function
King, Matthew G. , Barker, Michael S. , Kane, Nolan, C , Rieseberg, Loren H. .
Intragenic recombination in a homoploid hybrid species, Helianthus paradoxus.
Recombination is an important feature of genome stabilization following hybridization. The goal of our study is to investigate chimeric genesógenes that represent products of recombination between parental speciesí alleles.¬† Using expressed sequenced tag (EST) libraries we investigated the level of intragenic recombination within the homoploid hybrid Helianthus paradoxus, which is derived from a single origin via hybridization between the parental species H. annuus and H. petiolaris. We created DNA alignments of approximately 3300 orthologs from the three species and employed a maximum χ2 sliding window approach to search for chimeric alleles in H. paradoxus.¬† Twenty-nine putative chimeric genes were discovered, the majority of which contained exons from each of the parental species, with recombination inferred within an intron. Additionally, site-specific selection analyses indicate positive selection has played a role in the recent evolution of several of these genes.
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1 - University of British Columbia, Department of Botany, 3259-6270 University Blvd., Vancouver, BC, V6T1Z4, Canada
2 - University of British Columbia, Department of Botany, 3529-6270 University Blvd., Vancouver, BC, V6T 1Z4, Canada
3 - University of British Columbia, Department of Botany, 3529-6270 University Blvd, Vancouver, BC, V6T 1Z4, Canada
homoploid hybrid species.
Presentation Type: Oral Paper:Papers for Topics
Date: Monday, July 28th, 2008
Time: 3:15 PM