Tropical Biodiversity and Food Security
Jones, AMP , Murch, SJ , Saxena, PK .
In Vitro conservation and International distribution of breadfruit (Artocarpus altilis, Moracea).
In vivo germplasm collections are the traditional method of conservation of elite varieties of tropical crop species but require large areas of land, are vulnerable to infectious diseases and climate events and are difficult to maintain over long periods of time. Since 2002, efforts have been underway to replicate the in vivo collection of Breadfruit (Artocarpus altilis, Moraceae) at the National Tropical Botanical Garden, Hawaii, USA as a perpetuated in vitro collection. To date, 16 separate varieties have been established in vitro with 30 more varieties in the early stages of establishment. Lessons learned from this project include many insights into the differing cultural requirements of each variety. In general, shoot cultures can be induced on a solidified MS medium supplemented with 2 uM benzylaminopurine and 3 uM kinetin for 4 weeks. The rooting of the shoots is induced on MS medium with 5 uM indoleacetic acid (IAA) within 6 weeks. Robust plant growth can be accomplished in 2 L bioreactors each containing 100 ml MS medium enriched with 5 uM IAA. The plants after one month of growth in the bioreactor vessels attain a height of about 10 cm and the roots are approximately 30-40 cm in length. However, modification of these standard parameters is required for the different varieties to account for different capacities to accept growth regulator stimuli, rates of growth, sensitivities to antibiotics, and requirements for macro- or micro-nutrients. The optimization of in vitro production systems will ensure the conservation of the 173 traditional varieties of breadfruit and will allow for distribution of trees for food security worldwide.
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1 - UBC Okanagan, Biology, 3333 University Way, Kelowna, BC, V1V 1V7, Canada
2 - UBC Okanagan, Chemistry, 3333 University Way, Kelowna, BC, V1V 1V7, Canada
3 - University of Guelph, Department of Plant Agriculture, Guelph, Ontario, N1G 2W1, Canada
Presentation Type: Symposium or Colloquium Presentation
Date: Tuesday, July 29th, 2008
Time: 10:30 AM