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Abstract Detail


Wollemia nobilis: Modern Studies of an Ancient Plant

Murch, SJ [1], Saxena, PK [2].

Wollemi Pine (Wollemia nobilis) in Tissue Culture: Lessons from an Endangered Species.

The basic principles of plant tissue culture are well established and routine for most crop species but endangered species represent new challenges for in vitro production systems. By nature, endangered plant species have limited plant tissues for experimentation, often produce seed that is not viable and frequently have unusual requirements for plant growth regulators. In spite of the limitations, in vitro culture of Wollemia nobilis is required to provide plant material for research and conservation. A single cytokinin was not suitable for induction of shoots on Wollemi explants. Apical buds of Wollemia noblis were induced to proliferate on tissue culture media containing the two cytokinins benzylaminopurine (2 or 4 µM) and kinetin (4 µM). Shoots grow at a rate of about 0.2 cm per month and replication was possible from the de novo shoots. However transfer of de novo shoots to various rooting media did not result in the proliferation of roots. Difficulties in establishment of Wollemi pine in vitro illustrate many of the problems in application of standardized protocols to unusual species.


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1 - UBC Okanagan, Chemistry, 3333 University Way, Kelowna, BC, V1V 1V7, Canada
2 - University of Guelph, Department of Plant Agriculture, Guelph, Ontario, N1G 2W1, Canada

Keywords:
Wollemia nobilis
Endangered species
in vitro conservation.

Presentation Type: Symposium or Colloquium Presentation
Session: S3
Location: 207/SUB
Date: Monday, July 28th, 2008
Time: 11:00 AM
Number: S3005
Abstract ID:794


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