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Abstract Detail

Symbioses: Plant, Animal, and Microbe Interactions

Harrower, Emma [1], Lim, SeaRa [1], Kroeger, Paul [2], Berbee, Mary L. [1].

A Survey of the Genus Cortinarius in British Columbia.

The genus Cortinarius, with ~4000 described species is one of the most abundant, diverse and difficult genera of basidiomycete mushrooms. It includes an unknown and possibly large number of undescribed species. To establish a reference collection of sequences representing species of northwestern coastal North America, we sequenced the ribosomal ITS regions of as many as possible of 155 herbarium specimens collected from the year 2000 onwards from UBC and DAVFP herbaria; of 28 specimens collected by P. Kroeger; and of 82 other specimens newly collected in 2007. We are currently sequencing the ITS regions from 45 specimens collected by J. Ammirati. Our project is revealing conspecific or closely related collections as well-supported, tight phylogenetic clusters and is helping us to match North American species to sequences from European types. Using 146 sequences, a preliminary phylogenetic tree shows 69 different species-level clades. 44 of these (64%) were from well described species represented in Genbank. 13 of these (19%) only matched ectomycorrhizal root tips. 12 of these (17%) did not match anything in GenBank with >97% identity. Demonstrating how useful this DNA-barcoding has been, 25% (37/146) of the herbarium specimens were either misidentified and re-identified by following up on a GenBank match; or identified for the first time by reference to a GenBank sequence from a well-documented voucher specimen. By grouping collections by phylogenetic similarity, our study will facilitate identification of described western species and recognition and critical descriptions of new species.

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1 - University of British Columbia, Botany, 6270 University Boulevard, Vancouver, British Columbia, V6T 1Z4, Canada
2 - Mycological Consultant, 395 East 40th Avenue, Vancouver, British Columbia, V5W 1M1, Canada

DNA Barcoding.

Presentation Type: Oral Paper:Papers for Topics
Session: 40
Location: Blair A/Gage
Date: Tuesday, July 29th, 2008
Time: 11:00 AM
Number: 40005
Abstract ID:846

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