Developmental and Structural Section
Bakshi, Vibhu , Smith, Don W. , Maier, Camelia G.-A. .
In vitro Cultures of Morus alba Species for Enhancing Production of Phytoestrogens.
Enhancement of production of several secondary metabolites by tissue culture has been known for many years. Tissue cultures stimulate the production of or induce the biosynthesis of novel compounds not found in the mature plant. Tissue culture of Morus alba, family Moraceae, known to contain phytoestrogens, was established on plant-hormone supplemented Murashige and Skoog (MS) media. In vitro-obtained tissues, such as petiole and the stems from mature trees were the best explants for initiation and proliferation of callus. Callus proliferation from M. alba explants was obtained on MS medium containing NAA (1mg/ml) and BAP (0.5mg/ml). Comparison of phytoestrogens of Moraceae species from in vivo and in vitro tissue isolation were carried out. The estrogenic activities of callus extracts were assayed in an estrogen-responsive yeast system expressing the human estrogen receptor alpha. We also compared male and female extracts from in vivo and in vitro tissues. Isolation and characterization of phytoestrogens from above tissue extracts were carried out using SPE and HPLC chromatographic techniques. HPLC profiles of callus extracts were different from those of mature tree extracts. This indicates that phytoestrogens in in vitro cultures are different than those isolated from the corresponding mature tissues.
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1 - University of North Texas, Biological Sciences, UNT Box 305220, Denton, TX, 76203-5220, USA
2 - University of North Texas, Biology, Denton, Texas, 76203
3 - Texas Woman's University, Department of Biology, Denton, Texas, 76204-5799, USA
Presentation Type: Poster:Posters for Sections
Location: Ball Room & Party Room/SUB
Date: Monday, July 28th, 2008
Time: 12:30 PM